Md Amzad Hossain
University of Nizwa, Oman
Title: Isolation and characterization of antioxidant and antimicrobial agents from the leaves of Adenium obesum grown in Sultanate of Oman
Biography
Biography: Md Amzad Hossain
Abstract
A great impact in discovery of new drugs from medicinal plants has been witnessed in recent years as an effort to combat world health burden. Compounds with antioxidant and antimicrobial activities are in the centre of interest because of their influence on various diseases. Adenium obesum (A. obesum) has been used traditionally as a remedy for the treatment of venereal diseases, skin disease, dysentery, wound, headaches and muscle pain by local ethnic communities in Sultanate of Oman. However, there has been less interest in searching for active compounds from this plant. The aim of the present study was to isolate and identify the chemical constituents from locally grown A. obesum. Air dried leaves of A. obesum were extracted with methanol and the extract was succesively partitioned using hexane, chloroform, ethyl acetate, and n-butanol. Bioassay of these extracts showed chloroform extract to be the most active. It was therefore subjected to a series of chromatographic separation to afford two flavonoids. On the basis of chemical and spectral analysis their structures were elucidated as 5,7,3',4'-tetrahydroxyflavone and 3, 5,7,3',4',5'-hexahydroxyflavone. The results of antimicrobial study showed that the isolated pure compounds have significant antimicrobial activity.